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The two protein bands from 40 kDa to thirty kDa had been absent just after 24 h incubation at 45 °C. For the remainder of the bands, different concentrations of all a few proteases retained protein bands akin to their primary molar masses (Figure 6a,b). The existence of bands at very low molecular bodyweight in fish roe right before hydrolysis was surprising. Within the report by Ghelichi et al. [8], slight peaks of a comparatively reduced molecular body weight variety (three–fourteen kDa) were observed in defatted carp roe homogenate, most likely due to an autolytic approach transpiring in fish roe and/or the defatting approach bringing about the development of reduced-molecular-fat peptides.
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